4AEP
HCV-JFH1 NS5B POLYMERASE STRUCTURE AT 1.8 ANGSTROM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-02 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 100.970, 110.520, 115.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.244 - 1.800 |
| R-factor | 0.1886 |
| Rwork | 0.187 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xxd |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.971 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.240 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.120 | 1.200 |
| Number of reflections | 59618 | |
| <I/σ(I)> | 11.5 | 1.8 |
| Completeness [%] | 99.5 | 99 |
| Redundancy | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2 M SODIUM PHOSPHATE PH 6.5, 8-12% PEG 3350 |
