2XXD

HCV-JFH1 NS5B polymerase structure at 1.9 angstrom

Summary for 2XXD

• Entry DOI: 10.2210/pdb2xxd/pdb
• Related: 3I5K
• Descriptor: RNA-DIRECTED RNA POLYMERASE, PHOSPHATE ION (3 entities in total)
• Functional Keywords: transferase, hepacivirus, nonstructural proteins, replication, de novo initiation, priming
• Biological source: HEPATITIS C VIRUS (HCV)
• Cellular location: Core protein p21: Host endoplasmic reticulum membrane; Single-pass membrane protein (By similarity). Core protein p19: Virion (By similarity). Envelope glycoprotein E1: Virion membrane; Single-pass type I membrane protein (Potential). Envelope glycoprotein E2: Virion membrane; Single-pass type I membrane protein (Potential). p7: Host endoplasmic reticulum membrane; Multi-pass membrane protein. Protease NS2-3: Host endoplasmic reticulum membrane; Multi-pass membrane protein (Potential). Serine protease NS3: Host endoplasmic reticulum membrane; Peripheral membrane protein (Probable). Non-structural protein 4A: Host endoplasmic reticulum membrane; Single-pass type I membrane protein (Potential). Non-structural protein 4B: Host endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Non-structural protein 5A: Host endoplasmic reticulum membrane; Peripheral membrane protein (By similarity). RNA-directed RNA polymerase: Host endoplasmic reticulum membrane; Single-pass type I membrane protein (Potential): Q99IB8
• Total number of polymer chains: 1
• Total formula weight: 62971.82

Authors

Caillet-Saguy, C.,Bressanelli, S. (deposition date: 2010-11-10, release date: 2011-01-12, Last modification date: 2011-07-13)

• Primary citation: Schmitt, M.,Scrima, N.,Radujkovic, D.,Caillet-Saguy, C.,Simister, P.C.,Friebe, P.,Wicht, O.,Klein, R.,Bartenschlager, R.,Lohmann, V.,Bressanelli, S.; A Comprehensive Structure-Function Comparison of Hepatitis C Virus Strains Jfh1 and J6 Polymerases Reveals a Key Residue Stimulating Replication in Cell Culture Across Genotypes.; J.Virol., 85:2565-, 2011; PubMed: 21209117; DOI: 10.1128/JVI.02177-10

Experimental method

X-RAY DIFFRACTION (1.881 Å)