4AD7
Crystal structure of full-length N-glycosylated human glypican-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.250, 169.200, 151.710 |
| Unit cell angles | 90.00, 94.96, 90.00 |
Refinement procedure
| Resolution | 29.757 - 2.945 |
| R-factor | 0.2211 |
| Rwork | 0.218 |
| R-free | 0.27810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4acr |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.772 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: DEV_938)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.030 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.140 | 0.830 |
| Number of reflections | 49679 | |
| <I/σ(I)> | 15.4 | 3.4 |
| Completeness [%] | 99.4 | 94.1 |
| Redundancy | 8.9 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 12-14% PEG 6000, 0.1 M TRIS-HCL, 0.2 M CACL2, PH 8.0 |
