4ACP
Deactivation of human IgG1 Fc by endoglycosidase treatment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-14 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.290, 110.857, 77.508 |
| Unit cell angles | 90.00, 107.92, 90.00 |
Refinement procedure
| Resolution | 36.870 - 2.490 |
| R-factor | 0.1968 |
| Rwork | 0.195 |
| R-free | 0.23424 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2DTQ |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.238 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.300 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.110 | 0.670 |
| Number of reflections | 19399 | |
| <I/σ(I)> | 19.5 | 2.2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 8.4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 25% W/V POLYETHYLENE GLYCOL 1500, 0.1 M SPG SYSTEM BUFFER PH 4 |
