4AAP
Crystal structure of JMJD5 domain of human Lysine-specific demethylase 8 (KDM8) in complex with N-oxalylglycine (NOG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-22 |
Detector | ADSC CCD |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 68.575, 68.575, 269.003 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 89.670 - 2.600 |
R-factor | 0.20984 |
Rwork | 0.208 |
R-free | 0.25290 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.009 |
RMSD bond angle | 1.295 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.910 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.090 | 0.770 |
Number of reflections | 23473 | |
<I/σ(I)> | 10.2 | 2.2 |
Completeness [%] | 99.5 | 99.7 |
Redundancy | 7 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 16% PEG_3350, 0.007M NICL, 0M CDCL, 0.007M MGCL, 0.1M HEPES PH 7.3 |