4A69
Structure of HDAC3 bound to corepressor and inositol tetraphosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-05-18 |
Detector | PILATUS 2D HYBRID ARRAY |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 86.447, 118.634, 190.707 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 95.350 - 2.060 |
R-factor | 0.18955 |
Rwork | 0.187 |
R-free | 0.23617 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ew8 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.261 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.930 | 2.170 |
High resolution limit [Å] | 2.060 | 2.060 |
Rmerge | 0.100 | 0.330 |
Number of reflections | 49237 | |
<I/σ(I)> | 7.9 | 2.7 |
Completeness [%] | 85.9 | 68.9 |
Redundancy | 2.8 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.1 M HEPES PH 7.5 0.2 M NACL 10 % V/V PROPAN-2-OL |