4ZYO

Crystal Structure of Human Integral Membrane Stearoyl-CoA Desaturase with Substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	200
Detector technology	PIXEL
Collection date	2014-06-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.113
Spacegroup name	P 31 2 1
Unit cell lengths	134.108, 134.108, 112.561
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.520 - 3.250
R-factor	0.2424
Rwork	0.241
R-free	0.26360
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.941
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	116.000
High resolution limit [Å]	3.250
Number of reflections	18812
<I/σ(I)>	19
Completeness [%]	100.0
Redundancy	20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	100 mM Tris-HCl (pH 8.5), 30-35% (v/v) PEG400, 220 mM sodium citrate