4ZPK

Crystal Structure of the Heterodimeric HIF-2a:ARNT Complex with HRE DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97929
Spacegroup name	P 43
Unit cell lengths	65.746, 65.746, 245.929
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.873 - 3.600
R-factor	0.2381
Rwork	0.234
R-free	0.32130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zp4
RMSD bond length	0.004
RMSD bond angle	0.883
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.660
High resolution limit [Å]	3.600	9.750	3.600
Rmerge	0.097	0.046	0.722
Rmeas	0.114	0.055	0.852
Rpim	0.059	0.029	0.449
Total number of observations	45153
Number of reflections	12149
<I/σ(I)>	7.9
Completeness [%]	99.5	97.3	99.5
Redundancy	3.7	3.6	3.5
CC(1/2)		0.985	0.681

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	289	4% Tacsimate, pH 6.0, 10% PEG3350