4ZCH
Single-chain human APRIL-BAFF-BAFF Heterotrimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-05 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.99998 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 57.041, 117.865, 295.521 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.610 - 2.430 |
R-factor | 0.1788 |
Rwork | 0.177 |
R-free | 0.23410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1Q5X and 1KD7 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.220 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER-TNT (BUSTER 2.11.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 147.760 | 2.680 |
High resolution limit [Å] | 2.430 | 2.430 |
Rmerge | 0.079 | 0.505 |
Number of reflections | 36901 | |
<I/σ(I)> | 13.46 | |
Completeness [%] | 96.7 | 97.9 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | RESERVOIR SOLUTION : 14% PEG6000 , 1M LiCL , TRIS-HCL pH 8.50 |