4ZCF
Structural basis of asymmetric DNA methylation and ATP-triggered long-range diffusion by EcoP15I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97950 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 101.838, 101.838, 533.031 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.600 |
R-factor | 0.2206 |
Rwork | 0.218 |
R-free | 0.26250 |
Structure solution method | MIR |
RMSD bond length | 0.010 |
RMSD bond angle | 1.250 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.720 | |
Number of reflections | 85834 | |
<I/σ(I)> | 15.5 | 3.4 |
Completeness [%] | 97.3 | 94.5 |
Redundancy | 15.1 | 15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 5000 monomethylether, potassium acetate, HEPES |