4ZC7

Paromomycin bound to a leishmanial ribosomal A-site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9394
Spacegroup name	P 1
Unit cell lengths	31.690, 57.180, 47.700
Unit cell angles	78.92, 109.44, 123.61

Refinement procedure

Resolution	44.979 - 3.041
R-factor	0.1912
Rwork	0.183
R-free	0.26140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j7t
RMSD bond length	0.006
RMSD bond angle	1.230
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.000	45.000	3.120
High resolution limit [Å]	3.040	13.600	3.040
Rmerge	0.090	0.033	0.283
Rmeas	0.106	0.040	0.339
Total number of observations	15718
Number of reflections	5004	46	382
<I/σ(I)>	7.05	14.46	2.13
Completeness [%]	98.5	80.7	100
Redundancy	3.1		3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293.15	Sodium cacodylate pH 7.0, spermine tetrahydrochloride, 2-methyl-2,4-pentanediol (MPD), MgSO4, KCl.