4ZBW
Crystal structure of death effector domain of Caspase8 in Homo Sapiens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU MICROMAX-002+ |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-27 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.360, 52.106, 56.596 |
| Unit cell angles | 113.34, 116.89, 90.12 |
Refinement procedure
| Resolution | 29.220 - 2.200 |
| R-factor | 0.2082 |
| Rwork | 0.204 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bbr |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.800 |
| Data reduction software | CrystalClear (1.4) |
| Data scaling software | d*TREK (9.7) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.220 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.052 | 0.238 |
| Number of reflections | 22971 | |
| <I/σ(I)> | 9 | 2.6 |
| Completeness [%] | 96.6 | 93.8 |
| Redundancy | 1.83 | 1.59 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.5 | 293 | 0.2M sodium chloride, 25% PEG3350 |
