4Z87
Structure of the IMP dehydrogenase from Ashbya gossypii bound to GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-22 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00004 |
| Spacegroup name | P 4 |
| Unit cell lengths | 122.042, 122.042, 147.613 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.029 - 2.250 |
| R-factor | 0.1964 |
| Rwork | 0.195 |
| R-free | 0.22540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4z0g |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.737 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1839) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.050 | 2.310 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.110 | 1.650 |
| Number of reflections | 102432 | |
| <I/σ(I)> | 26.04 | 1.98 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.84 | 14.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 40% (v/v) 1,2-propanediol, 0.1 M sodium acetate, pH 4.5 |
