4Z1D
Structure of PEP and zinc bound KDO8PS from H.pylori
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-20 |
Detector | RIGAKU SATURN A200 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 140.300, 51.013, 78.547 |
Unit cell angles | 90.00, 104.37, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.650 |
R-factor | 0.18706 |
Rwork | 0.186 |
R-free | 0.21210 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.185 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.650 |
Number of reflections | 65566 |
<I/σ(I)> | 34.31 |
Completeness [%] | 99.2 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3350, HEPES, magnesium chloride |