4YYX
Crystal structure of the ZO-1 PDZ1 domain in complex with the 7-mer Claudin2 C-terminal tail
Replaces: 4OEQExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.351, 72.170, 76.958 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.322 - 1.790 |
| R-factor | 0.20086 |
| Rwork | 0.198 |
| R-free | 0.25356 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h3m |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.561 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.420 | |
| Number of reflections | 22067 | |
| <I/σ(I)> | 16.78 | 2.34 |
| Completeness [%] | 99.2 | 95.1 |
| Redundancy | 7.4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 2.0 M NAF, 0.1 M NAAC PH 4.6 |
