4YQX
Mouse IL-2 Bound to JES6-1 scFv Fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-01-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.976962 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 99.024, 99.024, 198.700 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.512 - 2.826 |
R-factor | 0.1712 |
Rwork | 0.170 |
R-free | 0.20430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b5i |
RMSD bond length | 0.005 |
RMSD bond angle | 0.857 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1951)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.920 |
High resolution limit [Å] | 2.820 | 6.070 | 2.820 |
Rmerge | 0.115 | 0.050 | 0.679 |
Rmeas | 0.121 | 0.053 | 0.728 |
Rpim | 0.038 | 0.017 | 0.257 |
Total number of observations | 268758 | ||
Number of reflections | 27586 | ||
<I/σ(I)> | 7.7 | ||
Completeness [%] | 99.9 | 100 | 99.3 |
Redundancy | 9.7 | 9.6 | 7.4 |
CC(1/2) | 0.999 | 0.842 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.4 | 295 | 0.1 M Bis-Tris propane, pH 6.4, 0.2 M sodium citrate, 19% PEG3350 |