4YOQ
Crystal Structure of MutY bound to its anti-substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.697, 84.594, 142.457 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.408 - 2.210 |
| R-factor | 0.1876 |
| Rwork | 0.184 |
| R-free | 0.24960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rrq |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.399 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 142.460 | 2.280 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 1.523 | |
| Number of reflections | 23671 | |
| <I/σ(I)> | 11.3 | 1.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.9 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 100 mM Tris, pH 8.0, 250 mM NaOAc, 29% (wt/vol) PEG 4000 |
