4YN0

Crystal structure of APP E2 domain in complex with DR6 CRD domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 61
Unit cell lengths	67.320, 67.320, 226.229
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.600 - 2.200
R-factor	0.2269
Rwork	0.226
R-free	0.24510
RMSD bond length	0.019
RMSD bond angle	1.250
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1760))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.076	0.652
Number of reflections	29375
<I/σ(I)>	14.63	2.34
Completeness [%]	99.9	99.7
Redundancy	6.7	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6	298	100mM Tris pH 8.6, 18% PEG 8000