4Y5X

Diabody 305 complex with EpoR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-06
Detector	PSI PILATUS 6M
Wavelength(s)	.9795
Spacegroup name	P 41 21 2
Unit cell lengths	239.540, 239.540, 132.569
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.940 - 3.150
R-factor	0.2035
Rwork	0.201
R-free	0.24060
RMSD bond length	0.003
RMSD bond angle	0.614
Data scaling software	Aimless (0.2.17)
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1839))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	24.940	24.940	3.230
High resolution limit [Å]	3.150	14.440	3.150
Rmerge	0.108	0.024	0.627
Rpim	0.061	0.015	0.360
Total number of observations	219210	1781	15487
Number of reflections	63411
<I/σ(I)>	10.1	30.4	1.6
Completeness [%]	95.2	70.4	97.1
Redundancy	3.5	3.3	3.4
CC(1/2)	0.994	1.000	0.705

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	sodium citrate, PEG 8000