4Y3C
I304V 3D polymerase mutant of EMCV
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-04-27 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 232.332, 140.775, 171.838 |
Unit cell angles | 90.00, 126.03, 90.00 |
Refinement procedure
Resolution | 138.960 - 3.200 |
R-factor | 0.22154 |
Rwork | 0.220 |
R-free | 0.24983 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nz0 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.773 |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.450 | 3.370 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.137 | 0.469 |
Number of reflections | 73423 | |
<I/σ(I)> | 5.2 | 2.2 |
Completeness [%] | 99.5 | 99.5 |
Redundancy | 2.9 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 4.0M AMMONIUM ACETATE, 0.1M SODIUM ACETATE PH 4.6 |