4Y30
Crystal structure of human protein arginine methyltransferase PRMT6 bound to SAH and EPZ020411
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.987 |
Spacegroup name | P 43 |
Unit cell lengths | 99.672, 99.672, 89.252 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.510 - 2.100 |
R-factor | 0.1818 |
Rwork | 0.180 |
R-free | 0.22080 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.386 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 99.670 |
High resolution limit [Å] | 2.100 |
Number of reflections | 50960 |
<I/σ(I)> | 8.3 |
Completeness [%] | 99.8 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | MgCl, MES buffer pH 6.5, Isopropanol, PEG 4000 |