4XXD
Crystal Structure of mid-region amyloid beta capture by solanezumab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 |
Unit cell lengths | 38.796, 73.555, 92.118 |
Unit cell angles | 109.91, 93.64, 93.31 |
Refinement procedure
Resolution | 46.560 - 2.410 |
R-factor | 0.2512 |
Rwork | 0.249 |
R-free | 0.28980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hix |
RMSD bond length | 0.011 |
RMSD bond angle | 1.290 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.560 | 2.510 |
High resolution limit [Å] | 2.410 | 2.410 |
Rmerge | 0.117 | 0.543 |
Number of reflections | 35968 | |
<I/σ(I)> | 8.2 | 2.3 |
Completeness [%] | 97.9 | 91.9 |
Redundancy | 3.9 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 292 | PEG 3350, sodium citrate |