4XKL
Crystal structure of NDP52 ZF2 in complex with mono-ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-01-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.950, 73.800, 39.700 |
| Unit cell angles | 90.00, 108.49, 90.00 |
Refinement procedure
| Resolution | 36.900 - 2.100 |
| R-factor | 0.19635 |
| Rwork | 0.194 |
| R-free | 0.24333 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.423 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.900 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.080 | 0.250 |
| Number of reflections | 12924 | |
| <I/σ(I)> | 9.58 | 4.6 |
| Completeness [%] | 96.3 | 97.48 |
| Redundancy | 3.33 | 3.34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2 M magnesium acetate tetrahydrate, 20% w/v Polyethylene glycol 3350 |
