4XI9
Human OGT in complex with UDP-5S-GlcNAc and substrate peptide (RBL2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.922 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 273.910, 273.910, 142.930 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 3.100 |
| R-factor | 0.20307 |
| Rwork | 0.203 |
| R-free | 0.22346 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.352 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 3.100 |
| Number of reflections | 108153 |
| <I/σ(I)> | 9.1 |
| Completeness [%] | 99.7 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.45 M potassium phosphate, 10 mM EDTA, 1% (w/v) xylitol |
