4XG7
Crystal structure of an inhibitor-bound Syk
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-29 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.901, 88.187, 40.982 |
| Unit cell angles | 90.00, 91.12, 90.00 |
Refinement procedure
| Resolution | 44.090 - 1.760 |
| R-factor | 0.22607 |
| Rwork | 0.223 |
| R-free | 0.24246 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xg2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.262 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Number of reflections | 27304 | |
| <I/σ(I)> | 58.15 | |
| Completeness [%] | 97.4 | |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 278 | 10~20%(v/v) PEG3350, 100 mM Tris-HCl |
