4XFT
Structure of IL-18 SER Mutant III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0,.97929 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.514, 55.042, 113.003 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.17899 |
| Rwork | 0.177 |
| R-free | 0.21802 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f62 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.295 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.562 | |
| Number of reflections | 24057 | |
| <I/σ(I)> | 30 | 3.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.2 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 35% PEG 3350, 0.1M MES, 5% DMSO |
