4XCU
Crystal Structure of FGFR4 with an Irreversible Inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9394 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.100, 58.110, 58.670 |
| Unit cell angles | 90.00, 94.42, 90.00 |
Refinement procedure
| Resolution | 19.510 - 1.710 |
| R-factor | 0.1956 |
| Rwork | 0.193 |
| R-free | 0.24270 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.672 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.800 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Number of reflections | 28521 | |
| <I/σ(I)> | 4.6 | 1.7 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 4.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 16% PEG 3350, 0.2M lithium sulfate, 0.1M bis-tris pH 4.5 |
