4X6Z
Yeast 20S proteasome in complex with PR-VI modulator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 133.350, 300.790, 144.580 |
Unit cell angles | 90.00, 112.11, 90.00 |
Refinement procedure
Resolution | 49.460 - 2.700 |
R-factor | 0.17957 |
Rwork | 0.177 |
R-free | 0.22192 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ryp |
RMSD bond length | 0.014 |
RMSD bond angle | 1.684 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.460 | 2.720 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 207816 | |
<I/σ(I)> | 6.5 | 2.9 |
Completeness [%] | 68.4 | 20.2 |
Redundancy | 1 | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 30mM magnesium acetate, 12% MPD, 0.1M MES pH 7.2 |