4X5F
ecDHFR complexed with folate and NADP+ at 0.1 MPa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-01-29 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.956, 59.926, 72.320 |
| Unit cell angles | 90.00, 102.82, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.15413 |
| Rwork | 0.152 |
| R-free | 0.18974 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.283 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 35866 |
| <I/σ(I)> | 20.7 |
| Completeness [%] | 95.9 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 6000, imidazole, calcium chloride |
