Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.87260 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 82.308, 82.308, 635.164 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.000 - 3.000 |
| R-factor | 0.22786 |
| Rwork | 0.226 |
| R-free | 0.27212 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j1j |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.406 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.200 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.645 | |
| Number of reflections | 45180 | |
| <I/σ(I)> | 15.1 | 3.3 |
| Completeness [%] | 98.4 | 90.5 |
| Redundancy | 13.2 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.05M MES, 0.2M KCL, 0.01M MGCL2, 5% PEG8000, PH 5.6, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K |
