4WTV
Crystal structure of the phosphatidylinositol 4-kinase IIbeta
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.28272 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 106.010, 106.010, 214.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.520 - 1.900 |
R-factor | 0.2104 |
Rwork | 0.209 |
R-free | 0.24040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pla |
RMSD bond length | 0.010 |
RMSD bond angle | 1.246 |
Data reduction software | XDS (January 10, 2014) |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.520 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.100 | |
Number of reflections | 96773 | |
<I/σ(I)> | 17.42 | 1.8 |
Completeness [%] | 100.0 | 99.94 |
Redundancy | 17.6 | 17.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 100 mM MES/Imidazole pH = 6.5, 10% w/v PEG 4000, 20% v/v glycerol, 20 mM 1,6-hexanediol, 20 mM 1-butanol, 20 mM 1,2-propanediol, 20 mM 2-propanol, 20 mM 1,4-butanediol, 20 mM 1,3-propanediol |