4WT8

Crystal Structure of bactobolin A bound to 70S ribosome-tRNA complex

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	90
Detector technology	PIXEL
Collection date	2013-06-14
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	214.210, 457.450, 639.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.908 - 3.400
R-factor	0.2062
Rwork	0.204
R-free	0.24120
RMSD bond length	0.009
RMSD bond angle	1.615
Data scaling software	SCALA (3.3.21)
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.908	49.908	3.580
High resolution limit [Å]	3.400	10.750	3.400
Rmerge		0.081	1.302
Rmeas	0.298
Rpim	0.112	0.028	0.608
Total number of observations	5690971	238813	610257
Number of reflections	841002
<I/σ(I)>	8.6	44.8	1.4
Completeness [%]	98.8	96.3	97.9
Redundancy	6.8	8.9	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	0.2M KSCN, 0.1M Tris-HAc pH7, 3.5-4.5%(w/v) PEG20K, 3.5- 4.5%(w/v) PEG550MME