4WSN

Crystal structure of the COP9 signalosome, a P1 crystal form

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-06-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0004
Spacegroup name	P 1
Unit cell lengths	150.638, 150.983, 336.720
Unit cell angles	92.34, 92.62, 119.88

Refinement procedure

Resolution	49.640 - 5.500
R-factor	0.2548
Rwork	0.254
R-free	0.28210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4d10 chains A-H
RMSD bond length	0.005
RMSD bond angle	0.839
Data reduction software	XDS (January 10, 2014)
Data scaling software	Aimless (0.3.11)
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.640	49.640	5.610
High resolution limit [Å]	5.500	28.040	5.500
Rmerge	0.072	0.025	1.329
Rpim	0.065	0.023	1.205
Total number of observations	153156	960	8216
Number of reflections	79098
<I/σ(I)>	5.4	20.2	0.6
Completeness [%]	94.9	78.8	88.7
Redundancy	1.9	1.9	1.9
CC(1/2)	0.997	0.998	0.166

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		273	CRYSTALS GROWN BY VAPOR DIFFUSION BY MIXING 9.3 MG/ML PROTEIN IN 50 MM HEPES PH 7.4, 200 MM NACL, 2 MM EQUALLY WITH 12% PEG 6000, 100 MM TRISODIUM CITRATE PH 5.4, 0.1 M LI2SO4.