4WRT

Crystal structure of Influenza B polymerase with bound vRNA promoter (form FluB2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 63 2 2
Unit cell lengths	207.370, 207.370, 345.690
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.657 - 2.700
R-factor	0.1753
Rwork	0.173
R-free	0.21140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wsa
RMSD bond length	0.003
RMSD bond angle	0.683
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1760))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.252	1.490
Number of reflections	120050
<I/σ(I)>	10.3	1.9
Completeness [%]	99.9	100
Redundancy	10.1	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	281	1 M LiCl, 10% PEG 6000 and 0.1 M bicine pH 9.0