4WRN
Crystal structure of the polymerization region of human uromodulin/Tamm-Horsfall protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-01-24 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97625 |
Spacegroup name | H 3 2 |
Unit cell lengths | 242.320, 242.320, 258.860 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.976 - 3.200 |
R-factor | 0.2222 |
Rwork | 0.221 |
R-free | 0.24590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3sex |
RMSD bond length | 0.004 |
RMSD bond angle | 0.840 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 3.314 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.088 | 1.462 |
Number of reflections | 47837 | |
<I/σ(I)> | 16.21 | 1.29 |
Completeness [%] | 99.4 | 99.52 |
Redundancy | 6.3 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 293 | Sodium/potassium tartrate, Tris-HCl |