4WQO
Structure of VHL-EloB-EloC-Cul2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 108.278, 108.278, 213.768 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.297 - 3.200 |
| R-factor | 0.2224 |
| Rwork | 0.221 |
| R-free | 0.24950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1LM8 and 2WZK |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.774 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.300 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.084 | |
| Number of reflections | 24321 | |
| <I/σ(I)> | 16.4 | 1.1 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 5.8 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 298 | 4M Na formate |
