4WND
Crystal structure of the TPR domain of LGN in complex with Frmpd4/Preso1 at 1.5 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2014-04-13 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.487, 64.786, 69.118 |
| Unit cell angles | 90.00, 102.33, 90.00 |
Refinement procedure
| Resolution | 46.790 - 1.500 |
| R-factor | 0.1636 |
| Rwork | 0.163 |
| R-free | 0.18240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wne |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.105 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP (11.0.05) |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 4.070 | 1.500 |
| Rmerge | 0.037 | 0.021 | 0.446 |
| Rmeas | 0.040 | 0.023 | 0.497 |
| Rpim | 0.014 | 0.008 | 0.219 |
| Total number of observations | 379458 | ||
| Number of reflections | 61319 | ||
| <I/σ(I)> | 21.9 | 2.724 | |
| Completeness [%] | 98.8 | 96.1 | 97.7 |
| Redundancy | 6.2 | 9.4 | 5 |
| CC(1/2) | 1.000 | 0.922 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 293 | 0.1 M BisTrispropane (pH 7.5), 0.2 M potassium thiocyanate, 20% PEG 3350 |
