4WIO
Crystal structure of the C89A GMP synthetase inactive mutant from Plasmodium falciparum in complex with glutamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.07812 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 76.920, 76.920, 204.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.030 - 3.150 |
| R-factor | 0.2652 |
| Rwork | 0.263 |
| R-free | 0.30620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uow |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.638 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.500 | 3.970 |
| High resolution limit [Å] | 3.150 | 3.470 |
| Rmerge | 0.376 | |
| Number of reflections | 11311 | |
| <I/σ(I)> | 10.1 | 3.92 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 0.1 M Tris-HCl pH 8.5, 22.5% (w/v) PEG 1000 and 10 mM Gln |
