4WEF
Structure of the Hemagglutinin-neuraminidase from Human parainfluenza virus type III: complex with difluorosialic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-20 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.000 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 218.668, 218.668, 109.769 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 77.460 - 2.500 |
R-factor | 0.19282 |
Rwork | 0.189 |
R-free | 0.25750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1v3c |
RMSD bond length | 0.014 |
RMSD bond angle | 1.800 |
Data reduction software | SCALEPACK |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 188.980 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.292 | 0.845 |
Number of reflections | 50876 | |
<I/σ(I)> | 7.5 | 1 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 8 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M Hepes, 1.0M (NH4)2SO4, 1.0M Li2SO4 |