4WD4
Crystal structure of human HO1 H25R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.080, 54.410, 122.280 |
| Unit cell angles | 90.00, 99.09, 90.00 |
Refinement procedure
| Resolution | 70.100 - 2.950 |
| R-factor | 0.20608 |
| Rwork | 0.203 |
| R-free | 0.25859 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n45 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.388 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.100 | 3.110 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Number of reflections | 20111 | |
| <I/σ(I)> | 8.7 | 2 |
| Completeness [%] | 97.4 | 92.4 |
| Redundancy | 6.6 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 100 mM HEPES, pH 7.4; 2.1 M (NH4)2SO4; 0.9% 1,6-hexane-diol; BIG_CHAP |
