4W5N
The Crystal Structure of Human Argonaute2 Bound to a Defined Guide RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.117, 108.870, 68.071 |
| Unit cell angles | 90.00, 106.93, 90.00 |
Refinement procedure
| Resolution | 38.980 - 2.900 |
| R-factor | 0.2192 |
| Rwork | 0.217 |
| R-free | 0.25330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ola |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.035 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.980 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.070 | |
| Number of reflections | 19381 | |
| <I/σ(I)> | 15.6 | 2.6 |
| Completeness [%] | 98.8 | 98.8 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | PEG, Tris, Isopropanol, Phenol, Magnesium |
