4W4U
Structure of yeast SAGA DUBm with Sgf73 Y57A mutant at 2.8 angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.034 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.739, 67.273, 137.145 |
| Unit cell angles | 90.00, 106.84, 90.00 |
Refinement procedure
| Resolution | 47.020 - 2.800 |
| R-factor | 0.18496 |
| Rwork | 0.182 |
| R-free | 0.24067 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mhs |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.371 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.020 |
| High resolution limit [Å] | 2.800 |
| Number of reflections | 34989 |
| <I/σ(I)> | 23.2 |
| Completeness [%] | 99.8 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 100mM Bis Tris,18% PEG3350, 100mM Ammonium Sulfate |
