4W4O
High-resolution crystal structure of Fc bound to its human receptor Fc-gamma-RI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.980, 126.500, 71.610 |
| Unit cell angles | 90.00, 118.95, 90.00 |
Refinement procedure
| Resolution | 39.700 - 1.800 |
| R-factor | 0.17497 |
| Rwork | 0.173 |
| R-free | 0.21501 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1L6X and 3RJD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.134 |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.700 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.074 | 0.870 |
| Number of reflections | 95656 | |
| <I/σ(I)> | 14.9 | 2.3 |
| Completeness [%] | 98.6 | 97.4 |
| Redundancy | 7.3 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.1M sodium acetate, 0.1 M zinc acetate, 4% 1,4-butanediol, 12% PEG 4,000 |
