4V8I

Crystal structure of YfiA bound to the 70S ribosome.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-04-02
Detector	PSI PILATUS 6M
Wavelength(s)	1.1000
Spacegroup name	P 21 21 21
Unit cell lengths	209.630, 449.300, 620.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.713 - 2.700
R-factor	0.2188
Rwork	0.217
R-free	0.25430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.500
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.713	49.713	2.770
High resolution limit [Å]	2.700	12.070	2.700
Rmerge	0.166	0.018	0.018
Number of reflections	1562621
<I/σ(I)>	10.89	51.46	0.82
Completeness [%]	98.7	97.9	94.5
Redundancy	7.57

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K