4V8A
The structure of thermorubin in complex with the 70S ribosome from Thermus thermophilus.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-04-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 209.370, 445.460, 619.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.290 - 3.200 |
| Rwork | 0.228 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.164 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.240 | 2.980 |
| High resolution limit [Å] | 2.900 | 3.150 | 2.900 |
| Rmerge | 0.717 | 2.650 | |
| Number of reflections | 1262956 | ||
| <I/σ(I)> | 1.73 | 0.65 | |
| Completeness [%] | 99.9 | 100 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293 | 2.9% PEG 20K, 9% MPD, 0.1M Tris-HCl pH 7.6, 175 mM L-Arginine, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
