4V4I

Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements.

This is a non-PDB format compatible entry.

Replaces:  1VS9Replaces:  2I1C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	I 4 2 2
Unit cell lengths	507.810, 507.810, 689.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.710
R-factor	0.349
Rwork	0.348
R-free	0.35300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdb entries 1TWT AND 1TWV
RMSD bond length	0.011
RMSD bond angle	1.393
Data reduction software	d*TREK
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.000	3.800
High resolution limit [Å]	3.700	3.700
Rmerge	0.149	0.467
Number of reflections	465894
Completeness [%]	99.3	96.6
Redundancy	5.4	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	MPD
2	1	1	H2O
3	1	2	MPD
4	1	2	H2O