4UXD
2-keto 3-deoxygluconate aldolase from Picrophilus torridus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-26 |
| Detector | SATURN 944PLUS |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 77.954, 100.159, 154.609 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.580 - 2.500 |
| R-factor | 0.1792 |
| Rwork | 0.175 |
| R-free | 0.25950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xky |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.179 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | BALBES |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.580 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.110 | 0.330 |
| Number of reflections | 36955 | |
| <I/σ(I)> | 13.25 | 2.65 |
| Completeness [%] | 86.6 | 83.4 |
| Redundancy | 3.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PROTEIN AT 4.2MG/ML MIXED 1:1 WITH (0.085 M HEPES SODIUM BUFFER PH 7.5, 8.5% V/V 2-PROPANOL, 17% W/V PEG 4000, 15% V/V GLYCEROL (ANHYDROUS)) |
