4UTB
Crystal structure of dengue 2 virus envelope glycoprotein in complex with the Fab fragment of the broadly neutralizing human antibody EDE2 A11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-11 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.779, 181.850, 204.823 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.850 |
| R-factor | 0.2311 |
| Rwork | 0.230 |
| R-free | 0.25670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h0t |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.180 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.220 |
| High resolution limit [Å] | 3.850 | 3.850 |
| Rmerge | 0.270 | 0.920 |
| Number of reflections | 21168 | |
| <I/σ(I)> | 5.8 | 1.4 |
| Completeness [%] | 98.3 | 98.3 |
| Redundancy | 4.83 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100MM HEPES PH 7.5 10% (W/V) PEG 6,000 5% (V/V) MPD |
