4UN5
Crystal structure of Cas9 bound to PAM-containing DNA target containing mismatches at positions 1-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-10 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.182, 68.072, 190.261 |
| Unit cell angles | 90.00, 111.37, 90.00 |
Refinement procedure
| Resolution | 48.349 - 2.400 |
| R-factor | 0.2165 |
| Rwork | 0.215 |
| R-free | 0.24650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4un3 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.686 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.350 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.090 | 0.370 |
| Number of reflections | 164018 | |
| <I/σ(I)> | 15.3 | 4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.6 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS-ACETATE PH 8.5, 0.3 M KSCN, 15% PEG (W/V) 3350 |
