4TO5
Structure basis of cellular dNTP regulation, SAMHD1-GTP-dTTP-dCTP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.459, 145.121, 98.233 |
| Unit cell angles | 90.00, 114.13, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.2717 |
| Rwork | 0.271 |
| R-free | 0.28460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bzb |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.422 |
| Data reduction software | HKL-2000 |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 7.590 | 2.800 |
| Rmerge | 0.188 | 0.098 | |
| Total number of observations | 253013 | ||
| Number of reflections | 56679 | ||
| <I/σ(I)> | 6.9 | ||
| Completeness [%] | 99.7 | 99.3 | 99.8 |
| Redundancy | 4.5 | 4.7 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 298 | SPG buffer, PEG 1500 |
